Investigation of BMP15 and GDF9 gene polymorphisms and their effects on litter size in Anatolian sheep breed Akkaraman

This study aimed to examine the polymorphism of BMP15 (FecX$^{G}$ and FecX$^{I}$) GDF9 (G1 G4) genes association between genotypes litter size in Akkaraman breed. Blood samples were taken from 100 ewes total. Genomic DNA was obtained whole blood. The PCR-RFLP technique employed determine polymorphisms under investigation. G1 only polymorphic among four loci analyzed. frequencies for GA GG 0.26 0.74, respectively, while A G allele 0.13 0.87, respectively. observed expected heterozygosity values 0.260 0.227, According chi-square analysis, tested population Hardy-Weinberg equilibrium (p > 0.05). analyses showed that genotypic structure did not significantly affect However, with one copy mutation produced more lambs than those without 0.05), suggesting heterozygous have a proclivity increase size, as evidenced by previous studies.